Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
42 |
2.9% |
| Arg[R] |
35 |
2.5% |
| Asn[N] |
104 |
7.3% |
| Asp[D] |
86 |
6.0% |
| Cys[C] |
22 |
1.5% |
| Gln[Q] |
63 |
4.4% |
| Glu[E] |
85 |
6.0% |
| Gly[G] |
28 |
2.0% |
| His[H] |
40 |
2.8% |
| Ile[I] |
143 |
10.0% |
| Leu[L] |
163 |
11.4% |
| Lys[K] |
115 |
8.1% |
| Met[M] |
37 |
2.6% |
| Phe[F] |
89 |
6.2% |
| Pro[P] |
45 |
3.2% |
| Ser[S] |
117 |
8.2% |
| Thr[T] |
73 |
5.1% |
| Trp[W] |
18 |
1.3% |
| Tyr[Y] |
55 |
3.9% |
| Val[V] |
67 |
4.7% |
| Total Length |
1427 |
|
| Instability index (II) |
36.43 |
| This classifies the protein as stable |
|
| Carbon |
7606 |
| Hydrogen |
11828 |
| Nitrogen |
1912 |
| Oxygen |
2182 |
| Sulfur |
59 |
| Total number of atoms |
23587 |
| Formula = C7606H11828N1912O2182S59 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
182325 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
180950 M-1 cm-1 |
| assuming all Cys residues are reduced |
180950 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
182325 M-1 cm-1 |
|
|
| Codon Bias |
0.042 |
| Codon Adaptation Index |
0.155 |
| Frequency of Optimal Codons |
0.458 |
| Hydropathicity of Protein |
-0.167 |
| Aromaticity Score |
0.114 |
|
