Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
67 |
5.5% |
| Arg[R] |
74 |
6.0% |
| Asn[N] |
92 |
7.5% |
| Asp[D] |
59 |
4.8% |
| Cys[C] |
4 |
0.3% |
| Gln[Q] |
39 |
3.2% |
| Glu[E] |
101 |
8.2% |
| Gly[G] |
44 |
3.6% |
| His[H] |
35 |
2.9% |
| Ile[I] |
59 |
4.8% |
| Leu[L] |
78 |
6.4% |
| Lys[K] |
108 |
8.8% |
| Met[M] |
17 |
1.4% |
| Phe[F] |
34 |
2.8% |
| Pro[P] |
91 |
7.4% |
| Ser[S] |
145 |
11.8% |
| Thr[T] |
79 |
6.4% |
| Trp[W] |
8 |
0.7% |
| Tyr[Y] |
37 |
3.0% |
| Val[V] |
55 |
4.5% |
| Total Length |
1226 |
|
| Instability index (II) |
58.63 |
| This classifies the protein as unstable |
|
| Carbon |
6022 |
| Hydrogen |
9573 |
| Nitrogen |
1765 |
| Oxygen |
1939 |
| Sulfur |
21 |
| Total number of atoms |
19320 |
| Formula = C6022H9573N1765O1939S21 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
99380 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
99130 M-1 cm-1 |
| assuming all Cys residues are reduced |
99130 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
99380 M-1 cm-1 |
|
|
| Codon Bias |
-0.001 |
| Codon Adaptation Index |
0.120 |
| Frequency of Optimal Codons |
0.403 |
| Hydropathicity of Protein |
-0.998 |
| Aromaticity Score |
0.064 |
|
