Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
32 |
7.0% |
| Arg[R] |
18 |
3.9% |
| Asn[N] |
18 |
3.9% |
| Asp[D] |
28 |
6.1% |
| Cys[C] |
0 |
0.0% |
| Gln[Q] |
17 |
3.7% |
| Glu[E] |
30 |
6.5% |
| Gly[G] |
32 |
7.0% |
| His[H] |
13 |
2.8% |
| Ile[I] |
21 |
4.6% |
| Leu[L] |
53 |
11.5% |
| Lys[K] |
32 |
7.0% |
| Met[M] |
8 |
1.7% |
| Phe[F] |
16 |
3.5% |
| Pro[P] |
22 |
4.8% |
| Ser[S] |
42 |
9.1% |
| Thr[T] |
18 |
3.9% |
| Trp[W] |
6 |
1.3% |
| Tyr[Y] |
22 |
4.8% |
| Val[V] |
32 |
7.0% |
| Total Length |
460 |
|
| Instability index (II) |
31.68 |
| This classifies the protein as stable |
|
| Carbon |
2317 |
| Hydrogen |
3609 |
| Nitrogen |
613 |
| Oxygen |
694 |
| Sulfur |
8 |
| Total number of atoms |
7241 |
| Formula = C2317H3609N613O694S8 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
65780 M-1 cm-1 |
|
|
| Codon Bias |
0.146 |
| Codon Adaptation Index |
0.186 |
| Frequency of Optimal Codons |
0.480 |
| Hydropathicity of Protein |
-0.333 |
| Aromaticity Score |
0.096 |
|