CIT2/YCR005C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 32 7.0%
Arg[R] 18 3.9%
Asn[N] 18 3.9%
Asp[D] 28 6.1%
Cys[C] 0 0.0%
Gln[Q] 17 3.7%
Glu[E] 30 6.5%
Gly[G] 32 7.0%
His[H] 13 2.8%
Ile[I] 21 4.6%
Leu[L] 53 11.5%
Lys[K] 32 7.0%
Met[M] 8 1.7%
Phe[F] 16 3.5%
Pro[P] 22 4.8%
Ser[S] 42 9.1%
Thr[T] 18 3.9%
Trp[W] 6 1.3%
Tyr[Y] 22 4.8%
Val[V] 32 7.0%
Total Length 460

Instability index
Instability index (II) 31.68
This classifies the protein as stable

Atomic composition
Carbon 2317
Hydrogen 3609
Nitrogen 613
Oxygen 694
Sulfur 8
Total number of atoms 7241
Formula = C2317H3609N613O694S8

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 65780 M-1 cm-1

Aliphatic index
Aliphatic index 89.87

Coding Region Translation Calculations
Codon Bias 0.146
Codon Adaptation Index 0.186
Frequency of Optimal Codons 0.480
Hydropathicity of Protein -0.333
Aromaticity Score 0.096