YCL074W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 18 5.8%
Arg[R] 13 4.2%
Asn[N] 11 3.6%
Asp[D] 16 5.2%
Cys[C] 5 1.6%
Gln[Q] 9 2.9%
Glu[E] 16 5.2%
Gly[G] 19 6.2%
His[H] 9 2.9%
Ile[I] 18 5.8%
Leu[L] 35 11.4%
Lys[K] 18 5.8%
Met[M] 6 1.9%
Phe[F] 9 2.9%
Pro[P] 19 6.2%
Ser[S] 26 8.4%
Thr[T] 22 7.1%
Trp[W] 3 1.0%
Tyr[Y] 18 5.8%
Val[V] 18 5.8%
Total Length 308

Instability index
Instability index (II) 39.26
This classifies the protein as stable

Atomic composition
Carbon 1555
Hydrogen 2428
Nitrogen 406
Oxygen 459
Sulfur 11
Total number of atoms 4859
Formula = C1555H2428N406O459S11

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 43570 M-1 cm-1
assuming NO Cys residues appear as half cystines 43320 M-1 cm-1
assuming all Cys residues are reduced 43320 M-1 cm-1
assuming all pairs of Cys residues form cystines 43570 M-1 cm-1

Aliphatic index
Aliphatic index 89.90

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000