HMLALPHA2/YCL067C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 6 2.9%
Arg[R] 12 5.7%
Asn[N] 18 8.6%
Asp[D] 10 4.8%
Cys[C] 2 1.0%
Gln[Q] 7 3.3%
Glu[E] 18 8.6%
Gly[G] 5 2.4%
His[H] 1 0.5%
Ile[I] 17 8.1%
Leu[L] 25 11.9%
Lys[K] 25 11.9%
Met[M] 4 1.9%
Phe[F] 6 2.9%
Pro[P] 8 3.8%
Ser[S] 16 7.6%
Thr[T] 17 8.1%
Trp[W] 2 1.0%
Tyr[Y] 3 1.4%
Val[V] 8 3.8%
Total Length 210

Instability index
Instability index (II) 43.20
This classifies the protein as unstable

Atomic composition
Carbon 1070
Hydrogen 1774
Nitrogen 300
Oxygen 328
Sulfur 6
Total number of atoms 3478
Formula = C1070H1774N300O328S6

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 15595 M-1 cm-1
assuming NO Cys residues appear as half cystines 15470 M-1 cm-1
assuming all Cys residues are reduced 15470 M-1 cm-1
assuming all pairs of Cys residues form cystines 15595 M-1 cm-1

Aliphatic index
Aliphatic index 91.90

Coding Region Translation Calculations
Codon Bias -0.034
Codon Adaptation Index 0.109
Frequency of Optimal Codons 0.397
Hydropathicity of Protein -0.667
Aromaticity Score 0.052