COS2/YBR302C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 15 4.0%
Arg[R] 14 3.7%
Asn[N] 21 5.5%
Asp[D] 10 2.6%
Cys[C] 9 2.4%
Gln[Q] 12 3.2%
Glu[E] 29 7.7%
Gly[G] 10 2.6%
His[H] 3 0.8%
Ile[I] 22 5.8%
Leu[L] 51 13.5%
Lys[K] 32 8.4%
Met[M] 11 2.9%
Phe[F] 32 8.4%
Pro[P] 14 3.7%
Ser[S] 29 7.7%
Thr[T] 11 2.9%
Trp[W] 16 4.2%
Tyr[Y] 14 3.7%
Val[V] 24 6.3%
Total Length 379

Instability index
Instability index (II) 38.15
This classifies the protein as stable

Atomic composition
Carbon 2119
Hydrogen 3212
Nitrogen 508
Oxygen 545
Sulfur 20
Total number of atoms 6404
Formula = C2119H3212N508O545S20

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 109360 M-1 cm-1
assuming NO Cys residues appear as half cystines 108860 M-1 cm-1
assuming all Cys residues are reduced 108860 M-1 cm-1
assuming all pairs of Cys residues form cystines 109360 M-1 cm-1

Aliphatic index
Aliphatic index 97.44

Coding Region Translation Calculations
Codon Bias 0.080
Codon Adaptation Index 0.134
Frequency of Optimal Codons 0.455
Hydropathicity of Protein 0.038
Aromaticity Score 0.164