YBR285W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 4 2.8%
Arg[R] 10 6.9%
Asn[N] 14 9.7%
Asp[D] 13 9.0%
Cys[C] 3 2.1%
Gln[Q] 3 2.1%
Glu[E] 10 6.9%
Gly[G] 4 2.8%
His[H] 3 2.1%
Ile[I] 11 7.6%
Leu[L] 10 6.9%
Lys[K] 13 9.0%
Met[M] 3 2.1%
Phe[F] 8 5.6%
Pro[P] 4 2.8%
Ser[S] 15 10.4%
Thr[T] 7 4.9%
Trp[W] 0 0.0%
Tyr[Y] 5 3.5%
Val[V] 4 2.8%
Total Length 144

Instability index
Instability index (II) 55.16
This classifies the protein as unstable

Atomic composition
Carbon 729
Hydrogen 1152
Nitrogen 210
Oxygen 235
Sulfur 6
Total number of atoms 2332
Formula = C729H1152N210O235S6

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 7575 M-1 cm-1
assuming NO Cys residues appear as half cystines 7450 M-1 cm-1
assuming all Cys residues are reduced 7450 M-1 cm-1
assuming all pairs of Cys residues form cystines 7575 M-1 cm-1

Aliphatic index
Aliphatic index 67.71

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000