Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
16 |
3.6% |
| Arg[R] |
23 |
5.2% |
| Asn[N] |
24 |
5.4% |
| Asp[D] |
51 |
11.5% |
| Cys[C] |
0 |
0.0% |
| Gln[Q] |
17 |
3.8% |
| Glu[E] |
51 |
11.5% |
| Gly[G] |
14 |
3.1% |
| His[H] |
7 |
1.6% |
| Ile[I] |
26 |
5.8% |
| Leu[L] |
39 |
8.8% |
| Lys[K] |
49 |
11.0% |
| Met[M] |
12 |
2.7% |
| Phe[F] |
10 |
2.2% |
| Pro[P] |
21 |
4.7% |
| Ser[S] |
25 |
5.6% |
| Thr[T] |
24 |
5.4% |
| Trp[W] |
3 |
0.7% |
| Tyr[Y] |
12 |
2.7% |
| Val[V] |
21 |
4.7% |
| Total Length |
445 |
|
| Instability index (II) |
54.45 |
| This classifies the protein as unstable |
|
| Carbon |
2252 |
| Hydrogen |
3609 |
| Nitrogen |
621 |
| Oxygen |
752 |
| Sulfur |
12 |
| Total number of atoms |
7246 |
| Formula = C2252H3609N621O752S12 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
34380 M-1 cm-1 |
|
|
| Codon Bias |
0.106 |
| Codon Adaptation Index |
0.184 |
| Frequency of Optimal Codons |
0.484 |
| Hydropathicity of Protein |
-1.076 |
| Aromaticity Score |
0.056 |
|
