RIF1/YBR275C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 64 3.3%
Arg[R] 79 4.1%
Asn[N] 151 7.9%
Asp[D] 107 5.6%
Cys[C] 19 1.0%
Gln[Q] 77 4.0%
Glu[E] 145 7.6%
Gly[G] 68 3.5%
His[H] 42 2.2%
Ile[I] 154 8.0%
Leu[L] 208 10.9%
Lys[K] 152 7.9%
Met[M] 41 2.1%
Phe[F] 69 3.6%
Pro[P] 89 4.6%
Ser[S] 186 9.7%
Thr[T] 96 5.0%
Trp[W] 13 0.7%
Tyr[Y] 45 2.3%
Val[V] 111 5.8%
Total Length 1916

Instability index
Instability index (II) 47.50
This classifies the protein as unstable

Atomic composition
Carbon 9653
Hydrogen 15490
Nitrogen 2630
Oxygen 2976
Sulfur 60
Total number of atoms 30809
Formula = C9653H15490N2630O2976S60

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 139675 M-1 cm-1
assuming NO Cys residues appear as half cystines 138550 M-1 cm-1
assuming all Cys residues are reduced 138550 M-1 cm-1
assuming all pairs of Cys residues form cystines 139675 M-1 cm-1

Aliphatic index
Aliphatic index 93.83

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000