Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
22 |
3.2% |
| Arg[R] |
47 |
6.8% |
| Asn[N] |
50 |
7.2% |
| Asp[D] |
30 |
4.3% |
| Cys[C] |
1 |
0.1% |
| Gln[Q] |
27 |
3.9% |
| Glu[E] |
45 |
6.5% |
| Gly[G] |
37 |
5.3% |
| His[H] |
26 |
3.7% |
| Ile[I] |
48 |
6.9% |
| Leu[L] |
57 |
8.2% |
| Lys[K] |
53 |
7.6% |
| Met[M] |
15 |
2.2% |
| Phe[F] |
13 |
1.9% |
| Pro[P] |
26 |
3.7% |
| Ser[S] |
76 |
11.0% |
| Thr[T] |
59 |
8.5% |
| Trp[W] |
10 |
1.4% |
| Tyr[Y] |
19 |
2.7% |
| Val[V] |
33 |
4.8% |
| Total Length |
694 |
|
| Instability index (II) |
47.40 |
| This classifies the protein as unstable |
|
| Carbon |
3441 |
| Hydrogen |
5541 |
| Nitrogen |
1027 |
| Oxygen |
1076 |
| Sulfur |
16 |
| Total number of atoms |
11101 |
| Formula = C3441H5541N1027O1076S16 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
83310 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
83310 M-1 cm-1 |
| assuming all Cys residues are reduced |
83310 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
83310 M-1 cm-1 |
|
|
| Codon Bias |
-0.010 |
| Codon Adaptation Index |
0.111 |
| Frequency of Optimal Codons |
0.404 |
| Hydropathicity of Protein |
-0.789 |
| Aromaticity Score |
0.061 |
|
