Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
58 |
5.1% |
| Arg[R] |
61 |
5.4% |
| Asn[N] |
61 |
5.4% |
| Asp[D] |
82 |
7.3% |
| Cys[C] |
9 |
0.8% |
| Gln[Q] |
46 |
4.1% |
| Glu[E] |
108 |
9.6% |
| Gly[G] |
51 |
4.5% |
| His[H] |
22 |
1.9% |
| Ile[I] |
63 |
5.6% |
| Leu[L] |
124 |
11.0% |
| Lys[K] |
119 |
10.5% |
| Met[M] |
20 |
1.8% |
| Phe[F] |
47 |
4.2% |
| Pro[P] |
41 |
3.6% |
| Ser[S] |
69 |
6.1% |
| Thr[T] |
49 |
4.3% |
| Trp[W] |
13 |
1.2% |
| Tyr[Y] |
39 |
3.5% |
| Val[V] |
47 |
4.2% |
| Total Length |
1129 |
|
| Instability index (II) |
40.89 |
| This classifies the protein as unstable |
|
| Carbon |
5839 |
| Hydrogen |
9243 |
| Nitrogen |
1595 |
| Oxygen |
1774 |
| Sulfur |
29 |
| Total number of atoms |
18480 |
| Formula = C5839H9243N1595O1774S29 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
130110 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
129610 M-1 cm-1 |
| assuming all Cys residues are reduced |
129610 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
130110 M-1 cm-1 |
|
|
| Codon Bias |
0.125 |
| Codon Adaptation Index |
0.192 |
| Frequency of Optimal Codons |
0.483 |
| Hydropathicity of Protein |
-0.742 |
| Aromaticity Score |
0.088 |
|
