Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
35 |
6.4% |
| Arg[R] |
22 |
4.1% |
| Asn[N] |
35 |
6.4% |
| Asp[D] |
24 |
4.4% |
| Cys[C] |
5 |
0.9% |
| Gln[Q] |
13 |
2.4% |
| Glu[E] |
25 |
4.6% |
| Gly[G] |
32 |
5.9% |
| His[H] |
12 |
2.2% |
| Ile[I] |
29 |
5.3% |
| Leu[L] |
43 |
7.9% |
| Lys[K] |
40 |
7.4% |
| Met[M] |
14 |
2.6% |
| Phe[F] |
35 |
6.4% |
| Pro[P] |
35 |
6.4% |
| Ser[S] |
36 |
6.6% |
| Thr[T] |
41 |
7.6% |
| Trp[W] |
2 |
0.4% |
| Tyr[Y] |
18 |
3.3% |
| Val[V] |
47 |
8.7% |
| Total Length |
543 |
|
| Instability index (II) |
31.38 |
| This classifies the protein as stable |
|
| Carbon |
2737 |
| Hydrogen |
4285 |
| Nitrogen |
723 |
| Oxygen |
785 |
| Sulfur |
19 |
| Total number of atoms |
8549 |
| Formula = C2737H4285N723O785S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
38070 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
37820 M-1 cm-1 |
| assuming all Cys residues are reduced |
37820 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
38070 M-1 cm-1 |
|
|
| Codon Bias |
0.243 |
| Codon Adaptation Index |
0.197 |
| Frequency of Optimal Codons |
0.552 |
| Hydropathicity of Protein |
-0.171 |
| Aromaticity Score |
0.101 |
|
