Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
36 |
5.4% |
| Arg[R] |
28 |
4.2% |
| Asn[N] |
80 |
11.9% |
| Asp[D] |
25 |
3.7% |
| Cys[C] |
4 |
0.6% |
| Gln[Q] |
52 |
7.7% |
| Glu[E] |
26 |
3.9% |
| Gly[G] |
34 |
5.1% |
| His[H] |
14 |
2.1% |
| Ile[I] |
29 |
4.3% |
| Leu[L] |
53 |
7.9% |
| Lys[K] |
25 |
3.7% |
| Met[M] |
29 |
4.3% |
| Phe[F] |
27 |
4.0% |
| Pro[P] |
47 |
7.0% |
| Ser[S] |
73 |
10.9% |
| Thr[T] |
42 |
6.2% |
| Trp[W] |
5 |
0.7% |
| Tyr[Y] |
18 |
2.7% |
| Val[V] |
25 |
3.7% |
| Total Length |
672 |
|
| Instability index (II) |
53.31 |
| This classifies the protein as unstable |
|
| Carbon |
3244 |
| Hydrogen |
5072 |
| Nitrogen |
946 |
| Oxygen |
1040 |
| Sulfur |
33 |
| Total number of atoms |
10335 |
| Formula = C3244H5072N946O1040S33 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
54570 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
54320 M-1 cm-1 |
| assuming all Cys residues are reduced |
54320 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
54570 M-1 cm-1 |
|
|
| Codon Bias |
0.036 |
| Codon Adaptation Index |
0.119 |
| Frequency of Optimal Codons |
0.422 |
| Hydropathicity of Protein |
-0.701 |
| Aromaticity Score |
0.074 |
|
