Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
127 |
6.9% |
| Arg[R] |
74 |
4.0% |
| Asn[N] |
82 |
4.5% |
| Asp[D] |
97 |
5.3% |
| Cys[C] |
31 |
1.7% |
| Gln[Q] |
60 |
3.3% |
| Glu[E] |
127 |
6.9% |
| Gly[G] |
144 |
7.8% |
| His[H] |
23 |
1.3% |
| Ile[I] |
127 |
6.9% |
| Leu[L] |
156 |
8.5% |
| Lys[K] |
117 |
6.4% |
| Met[M] |
26 |
1.4% |
| Phe[F] |
72 |
3.9% |
| Pro[P] |
102 |
5.6% |
| Ser[S] |
144 |
7.8% |
| Thr[T] |
95 |
5.2% |
| Trp[W] |
22 |
1.2% |
| Tyr[Y] |
65 |
3.5% |
| Val[V] |
144 |
7.8% |
| Total Length |
1835 |
|
| Instability index (II) |
37.40 |
| This classifies the protein as stable |
|
| Carbon |
9042 |
| Hydrogen |
14218 |
| Nitrogen |
2384 |
| Oxygen |
2730 |
| Sulfur |
57 |
| Total number of atoms |
28431 |
| Formula = C9042H14218N2384O2730S57 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
219725 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
217850 M-1 cm-1 |
| assuming all Cys residues are reduced |
217850 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
219725 M-1 cm-1 |
|
|
| Codon Bias |
0.199 |
| Codon Adaptation Index |
0.194 |
| Frequency of Optimal Codons |
0.522 |
| Hydropathicity of Protein |
-0.177 |
| Aromaticity Score |
0.087 |
|
