LDH1/YBR204C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 18 4.8%
Arg[R] 14 3.7%
Asn[N] 22 5.9%
Asp[D] 24 6.4%
Cys[C] 9 2.4%
Gln[Q] 18 4.8%
Glu[E] 25 6.7%
Gly[G] 18 4.8%
His[H] 10 2.7%
Ile[I] 23 6.1%
Leu[L] 40 10.7%
Lys[K] 30 8.0%
Met[M] 6 1.6%
Phe[F] 23 6.1%
Pro[P] 15 4.0%
Ser[S] 19 5.1%
Thr[T] 14 3.7%
Trp[W] 8 2.1%
Tyr[Y] 10 2.7%
Val[V] 29 7.7%
Total Length 375

Instability index
Instability index (II) 34.37
This classifies the protein as stable

Atomic composition
Carbon 1966
Hydrogen 3043
Nitrogen 515
Oxygen 557
Sulfur 15
Total number of atoms 6096
Formula = C1966H3043N515O557S15

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 59400 M-1 cm-1
assuming NO Cys residues appear as half cystines 58900 M-1 cm-1
assuming all Cys residues are reduced 58900 M-1 cm-1
assuming all pairs of Cys residues form cystines 59400 M-1 cm-1

Aliphatic index
Aliphatic index 92.75

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000