MCM7/YBR202W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 54 6.4%
Arg[R] 46 5.4%
Asn[N] 45 5.3%
Asp[D] 65 7.7%
Cys[C] 12 1.4%
Gln[Q] 41 4.9%
Glu[E] 55 6.5%
Gly[G] 38 4.5%
His[H] 10 1.2%
Ile[I] 52 6.2%
Leu[L] 90 10.7%
Lys[K] 51 6.0%
Met[M] 25 3.0%
Phe[F] 29 3.4%
Pro[P] 35 4.1%
Ser[S] 60 7.1%
Thr[T] 55 6.5%
Trp[W] 1 0.1%
Tyr[Y] 25 3.0%
Val[V] 56 6.6%
Total Length 845

Instability index
Instability index (II) 43.16
This classifies the protein as unstable

Atomic composition
Carbon 4165
Hydrogen 6705
Nitrogen 1141
Oxygen 1312
Sulfur 37
Total number of atoms 13360
Formula = C4165H6705N1141O1312S37

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 42010 M-1 cm-1
assuming NO Cys residues appear as half cystines 41260 M-1 cm-1
assuming all Cys residues are reduced 42750 M-1 cm-1
assuming all pairs of Cys residues form cystines 43500 M-1 cm-1

Aliphatic index
Aliphatic index 91.15

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000