Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
36 |
12.9% |
| Arg[R] |
8 |
2.9% |
| Asn[N] |
3 |
1.1% |
| Asp[D] |
13 |
4.6% |
| Cys[C] |
1 |
0.4% |
| Gln[Q] |
6 |
2.1% |
| Glu[E] |
16 |
5.7% |
| Gly[G] |
20 |
7.1% |
| His[H] |
5 |
1.8% |
| Ile[I] |
25 |
8.9% |
| Leu[L] |
29 |
10.4% |
| Lys[K] |
14 |
5.0% |
| Met[M] |
17 |
6.1% |
| Phe[F] |
17 |
6.1% |
| Pro[P] |
2 |
0.7% |
| Ser[S] |
26 |
9.3% |
| Thr[T] |
15 |
5.4% |
| Trp[W] |
2 |
0.7% |
| Tyr[Y] |
7 |
2.5% |
| Val[V] |
18 |
6.4% |
| Total Length |
280 |
|
| Instability index (II) |
30.79 |
| This classifies the protein as stable |
|
| Carbon |
1372 |
| Hydrogen |
2183 |
| Nitrogen |
339 |
| Oxygen |
396 |
| Sulfur |
18 |
| Total number of atoms |
4308 |
| Formula = C1372H2183N339O396S18 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
21430 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
21430 M-1 cm-1 |
| assuming all Cys residues are reduced |
21430 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
21430 M-1 cm-1 |
|
|
| Codon Bias |
0.156 |
| Codon Adaptation Index |
0.162 |
| Frequency of Optimal Codons |
0.490 |
| Hydropathicity of Protein |
0.545 |
| Aromaticity Score |
0.093 |
|
