Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
40 |
5.4% |
| Arg[R] |
20 |
2.7% |
| Asn[N] |
42 |
5.7% |
| Asp[D] |
36 |
4.9% |
| Cys[C] |
2 |
0.3% |
| Gln[Q] |
40 |
5.4% |
| Glu[E] |
50 |
6.8% |
| Gly[G] |
39 |
5.3% |
| His[H] |
12 |
1.6% |
| Ile[I] |
32 |
4.3% |
| Leu[L] |
57 |
7.7% |
| Lys[K] |
73 |
9.9% |
| Met[M] |
15 |
2.0% |
| Phe[F] |
23 |
3.1% |
| Pro[P] |
37 |
5.0% |
| Ser[S] |
102 |
13.8% |
| Thr[T] |
67 |
9.1% |
| Trp[W] |
11 |
1.5% |
| Tyr[Y] |
9 |
1.2% |
| Val[V] |
33 |
4.5% |
| Total Length |
740 |
|
| Instability index (II) |
46.65 |
| This classifies the protein as unstable |
|
| Carbon |
3538 |
| Hydrogen |
5678 |
| Nitrogen |
990 |
| Oxygen |
1173 |
| Sulfur |
17 |
| Total number of atoms |
11396 |
| Formula = C3538H5678N990O1173S17 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
74035 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
73910 M-1 cm-1 |
| assuming all Cys residues are reduced |
73910 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
74035 M-1 cm-1 |
|
|
| Codon Bias |
0.117 |
| Codon Adaptation Index |
0.157 |
| Frequency of Optimal Codons |
0.476 |
| Hydropathicity of Protein |
-0.753 |
| Aromaticity Score |
0.058 |
|
