Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
54 |
7.8% |
| Arg[R] |
33 |
4.8% |
| Asn[N] |
37 |
5.3% |
| Asp[D] |
55 |
7.9% |
| Cys[C] |
4 |
0.6% |
| Gln[Q] |
17 |
2.5% |
| Glu[E] |
55 |
7.9% |
| Gly[G] |
34 |
4.9% |
| His[H] |
9 |
1.3% |
| Ile[I] |
41 |
5.9% |
| Leu[L] |
54 |
7.8% |
| Lys[K] |
63 |
9.1% |
| Met[M] |
10 |
1.4% |
| Phe[F] |
28 |
4.0% |
| Pro[P] |
31 |
4.5% |
| Ser[S] |
43 |
6.2% |
| Thr[T] |
48 |
6.9% |
| Trp[W] |
4 |
0.6% |
| Tyr[Y] |
21 |
3.0% |
| Val[V] |
52 |
7.5% |
| Total Length |
693 |
|
| Instability index (II) |
31.99 |
| This classifies the protein as stable |
|
| Carbon |
3441 |
| Hydrogen |
5479 |
| Nitrogen |
931 |
| Oxygen |
1080 |
| Sulfur |
14 |
| Total number of atoms |
10945 |
| Formula = C3441H5479N931O1080S14 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
53540 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
53290 M-1 cm-1 |
| assuming all Cys residues are reduced |
53290 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
53540 M-1 cm-1 |
|
|
| Codon Bias |
0.134 |
| Codon Adaptation Index |
0.192 |
| Frequency of Optimal Codons |
0.492 |
| Hydropathicity of Protein |
-0.500 |
| Aromaticity Score |
0.076 |
|
