Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
46 |
4.2% |
| Arg[R] |
54 |
4.9% |
| Asn[N] |
86 |
7.9% |
| Asp[D] |
99 |
9.0% |
| Cys[C] |
19 |
1.7% |
| Gln[Q] |
27 |
2.5% |
| Glu[E] |
78 |
7.1% |
| Gly[G] |
28 |
2.6% |
| His[H] |
29 |
2.7% |
| Ile[I] |
57 |
5.2% |
| Leu[L] |
112 |
10.2% |
| Lys[K] |
81 |
7.4% |
| Met[M] |
25 |
2.3% |
| Phe[F] |
62 |
5.7% |
| Pro[P] |
35 |
3.2% |
| Ser[S] |
94 |
8.6% |
| Thr[T] |
61 |
5.6% |
| Trp[W] |
7 |
0.6% |
| Tyr[Y] |
41 |
3.7% |
| Val[V] |
53 |
4.8% |
| Total Length |
1094 |
|
| Instability index (II) |
49.05 |
| This classifies the protein as unstable |
|
| Carbon |
5609 |
| Hydrogen |
8707 |
| Nitrogen |
1515 |
| Oxygen |
1758 |
| Sulfur |
44 |
| Total number of atoms |
17633 |
| Formula = C5609H8707N1515O1758S44 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
100715 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
99590 M-1 cm-1 |
| assuming all Cys residues are reduced |
99590 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
100715 M-1 cm-1 |
|
|
| Codon Bias |
-0.025 |
| Codon Adaptation Index |
0.140 |
| Frequency of Optimal Codons |
0.402 |
| Hydropathicity of Protein |
-0.599 |
| Aromaticity Score |
0.101 |
|
