Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
36 |
7.0% |
| Arg[R] |
33 |
6.4% |
| Asn[N] |
23 |
4.4% |
| Asp[D] |
36 |
7.0% |
| Cys[C] |
1 |
0.2% |
| Gln[Q] |
16 |
3.1% |
| Glu[E] |
44 |
8.5% |
| Gly[G] |
37 |
7.2% |
| His[H] |
7 |
1.4% |
| Ile[I] |
42 |
8.1% |
| Leu[L] |
42 |
8.1% |
| Lys[K] |
26 |
5.0% |
| Met[M] |
12 |
2.3% |
| Phe[F] |
20 |
3.9% |
| Pro[P] |
27 |
5.2% |
| Ser[S] |
33 |
6.4% |
| Thr[T] |
31 |
6.0% |
| Trp[W] |
1 |
0.2% |
| Tyr[Y] |
19 |
3.7% |
| Val[V] |
31 |
6.0% |
| Total Length |
517 |
|
| Instability index (II) |
33.72 |
| This classifies the protein as stable |
|
| Carbon |
2556 |
| Hydrogen |
4052 |
| Nitrogen |
696 |
| Oxygen |
800 |
| Sulfur |
13 |
| Total number of atoms |
8117 |
| Formula = C2556H4052N696O800S13 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
33810 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
33810 M-1 cm-1 |
| assuming all Cys residues are reduced |
33810 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
33810 M-1 cm-1 |
|
|
| Codon Bias |
0.474 |
| Codon Adaptation Index |
0.390 |
| Frequency of Optimal Codons |
0.687 |
| Hydropathicity of Protein |
-0.378 |
| Aromaticity Score |
0.077 |
|
