Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
91 |
6.5% |
| Arg[R] |
61 |
4.4% |
| Asn[N] |
80 |
5.7% |
| Asp[D] |
97 |
7.0% |
| Cys[C] |
14 |
1.0% |
| Gln[Q] |
42 |
3.0% |
| Glu[E] |
75 |
5.4% |
| Gly[G] |
90 |
6.5% |
| His[H] |
26 |
1.9% |
| Ile[I] |
81 |
5.8% |
| Leu[L] |
142 |
10.2% |
| Lys[K] |
86 |
6.2% |
| Met[M] |
21 |
1.5% |
| Phe[F] |
61 |
4.4% |
| Pro[P] |
74 |
5.3% |
| Ser[S] |
95 |
6.8% |
| Thr[T] |
86 |
6.2% |
| Trp[W] |
18 |
1.3% |
| Tyr[Y] |
51 |
3.7% |
| Val[V] |
101 |
7.3% |
| Total Length |
1392 |
|
| Instability index (II) |
28.57 |
| This classifies the protein as stable |
|
| Carbon |
6979 |
| Hydrogen |
10903 |
| Nitrogen |
1853 |
| Oxygen |
2091 |
| Sulfur |
35 |
| Total number of atoms |
21861 |
| Formula = C6979H10903N1853O2091S35 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
175865 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
174990 M-1 cm-1 |
| assuming all Cys residues are reduced |
174990 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
175865 M-1 cm-1 |
|
|
| Codon Bias |
0.195 |
| Codon Adaptation Index |
0.212 |
| Frequency of Optimal Codons |
0.518 |
| Hydropathicity of Protein |
-0.257 |
| Aromaticity Score |
0.093 |
|
