Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
33 |
4.2% |
| Arg[R] |
49 |
6.2% |
| Asn[N] |
51 |
6.5% |
| Asp[D] |
55 |
7.0% |
| Cys[C] |
16 |
2.0% |
| Gln[Q] |
36 |
4.6% |
| Glu[E] |
53 |
6.7% |
| Gly[G] |
32 |
4.1% |
| His[H] |
19 |
2.4% |
| Ile[I] |
51 |
6.5% |
| Leu[L] |
72 |
9.1% |
| Lys[K] |
67 |
8.5% |
| Met[M] |
18 |
2.3% |
| Phe[F] |
34 |
4.3% |
| Pro[P] |
28 |
3.5% |
| Ser[S] |
50 |
6.3% |
| Thr[T] |
50 |
6.3% |
| Trp[W] |
9 |
1.1% |
| Tyr[Y] |
22 |
2.8% |
| Val[V] |
45 |
5.7% |
| Total Length |
790 |
|
| Instability index (II) |
52.27 |
| This classifies the protein as unstable |
|
| Carbon |
4036 |
| Hydrogen |
6418 |
| Nitrogen |
1138 |
| Oxygen |
1216 |
| Sulfur |
34 |
| Total number of atoms |
12842 |
| Formula = C4036H6418N1138O1216S34 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
83280 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
82280 M-1 cm-1 |
| assuming all Cys residues are reduced |
82280 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
83280 M-1 cm-1 |
|
|
| Codon Bias |
0.103 |
| Codon Adaptation Index |
0.163 |
| Frequency of Optimal Codons |
0.477 |
| Hydropathicity of Protein |
-0.599 |
| Aromaticity Score |
0.082 |
|
