Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
27 |
5.0% |
| Arg[R] |
15 |
2.8% |
| Asn[N] |
34 |
6.4% |
| Asp[D] |
38 |
7.1% |
| Cys[C] |
5 |
0.9% |
| Gln[Q] |
19 |
3.6% |
| Glu[E] |
37 |
6.9% |
| Gly[G] |
42 |
7.9% |
| His[H] |
14 |
2.6% |
| Ile[I] |
38 |
7.1% |
| Leu[L] |
52 |
9.7% |
| Lys[K] |
30 |
5.6% |
| Met[M] |
7 |
1.3% |
| Phe[F] |
15 |
2.8% |
| Pro[P] |
13 |
2.4% |
| Ser[S] |
55 |
10.3% |
| Thr[T] |
27 |
5.0% |
| Trp[W] |
12 |
2.2% |
| Tyr[Y] |
15 |
2.8% |
| Val[V] |
40 |
7.5% |
| Total Length |
535 |
|
| Instability index (II) |
28.80 |
| This classifies the protein as stable |
|
| Carbon |
2617 |
| Hydrogen |
4089 |
| Nitrogen |
703 |
| Oxygen |
836 |
| Sulfur |
12 |
| Total number of atoms |
8257 |
| Formula = C2617H4089N703O836S12 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
88600 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
88350 M-1 cm-1 |
| assuming all Cys residues are reduced |
88350 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
88600 M-1 cm-1 |
|
|
| Codon Bias |
-0.004 |
| Codon Adaptation Index |
0.108 |
| Frequency of Optimal Codons |
0.409 |
| Hydropathicity of Protein |
-0.290 |
| Aromaticity Score |
0.079 |
|
