Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
35 |
5.1% |
| Arg[R] |
33 |
4.8% |
| Asn[N] |
47 |
6.8% |
| Asp[D] |
50 |
7.2% |
| Cys[C] |
7 |
1.0% |
| Gln[Q] |
34 |
4.9% |
| Glu[E] |
50 |
7.2% |
| Gly[G] |
24 |
3.5% |
| His[H] |
13 |
1.9% |
| Ile[I] |
48 |
6.9% |
| Leu[L] |
58 |
8.4% |
| Lys[K] |
67 |
9.7% |
| Met[M] |
12 |
1.7% |
| Phe[F] |
26 |
3.8% |
| Pro[P] |
28 |
4.1% |
| Ser[S] |
74 |
10.7% |
| Thr[T] |
29 |
4.2% |
| Trp[W] |
3 |
0.4% |
| Tyr[Y] |
22 |
3.2% |
| Val[V] |
31 |
4.5% |
| Total Length |
691 |
|
| Instability index (II) |
51.56 |
| This classifies the protein as unstable |
|
| Carbon |
3454 |
| Hydrogen |
5513 |
| Nitrogen |
967 |
| Oxygen |
1098 |
| Sulfur |
19 |
| Total number of atoms |
11051 |
| Formula = C3454H5513N967O1098S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
49655 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
49280 M-1 cm-1 |
| assuming all Cys residues are reduced |
49280 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
49655 M-1 cm-1 |
|
|
| Codon Bias |
-0.060 |
| Codon Adaptation Index |
0.108 |
| Frequency of Optimal Codons |
0.382 |
| Hydropathicity of Protein |
-0.734 |
| Aromaticity Score |
0.074 |
|
