VPS15/YBR097W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 49 3.4%
Arg[R] 62 4.3%
Asn[N] 106 7.3%
Asp[D] 77 5.3%
Cys[C] 29 2.0%
Gln[Q] 51 3.5%
Glu[E] 85 5.8%
Gly[G] 49 3.4%
His[H] 26 1.8%
Ile[I] 137 9.4%
Leu[L] 157 10.8%
Lys[K] 91 6.3%
Met[M] 24 1.7%
Phe[F] 80 5.5%
Pro[P] 53 3.6%
Ser[S] 140 9.6%
Thr[T] 84 5.8%
Trp[W] 11 0.8%
Tyr[Y] 54 3.7%
Val[V] 89 6.1%
Total Length 1454

Instability index
Instability index (II) 40.57
This classifies the protein as unstable

Atomic composition
Carbon 7495
Hydrogen 11807
Nitrogen 1951
Oxygen 2214
Sulfur 53
Total number of atoms 23520
Formula = C7495H11807N1951O2214S53

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 142710 M-1 cm-1
assuming NO Cys residues appear as half cystines 140960 M-1 cm-1
assuming all Cys residues are reduced 140960 M-1 cm-1
assuming all pairs of Cys residues form cystines 142710 M-1 cm-1

Aliphatic index
Aliphatic index 99.98

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000