RXT2/YBR095C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 18 4.2%
Arg[R] 27 6.3%
Asn[N] 41 9.5%
Asp[D] 27 6.3%
Cys[C] 0 0.0%
Gln[Q] 15 3.5%
Glu[E] 45 10.5%
Gly[G] 19 4.4%
His[H] 5 1.2%
Ile[I] 39 9.1%
Leu[L] 42 9.8%
Lys[K] 23 5.3%
Met[M] 7 1.6%
Phe[F] 11 2.6%
Pro[P] 15 3.5%
Ser[S] 42 9.8%
Thr[T] 23 5.3%
Trp[W] 1 0.2%
Tyr[Y] 9 2.1%
Val[V] 21 4.9%
Total Length 430

Instability index
Instability index (II) 60.97
This classifies the protein as unstable

Atomic composition
Carbon 2104
Hydrogen 3409
Nitrogen 601
Oxygen 705
Sulfur 7
Total number of atoms 6826
Formula = C2104H3409N601O705S7

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 18910 M-1 cm-1

Aliphatic index
Aliphatic index 91.81

Coding Region Translation Calculations
Codon Bias 0.099
Codon Adaptation Index 0.146
Frequency of Optimal Codons 0.472
Hydropathicity of Protein -0.626
Aromaticity Score 0.049