ECM33/YBR078W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 42 9.8%
Arg[R] 3 0.7%
Asn[N] 38 8.9%
Asp[D] 19 4.4%
Cys[C] 4 0.9%
Gln[Q] 16 3.7%
Glu[E] 10 2.3%
Gly[G] 29 6.8%
His[H] 0 0.0%
Ile[I] 27 6.3%
Leu[L] 42 9.8%
Lys[K] 19 4.4%
Met[M] 4 0.9%
Phe[F] 20 4.7%
Pro[P] 5 1.2%
Ser[S] 85 19.8%
Thr[T] 37 8.6%
Trp[W] 1 0.2%
Tyr[Y] 1 0.2%
Val[V] 27 6.3%
Total Length 429

Instability index
Instability index (II) 32.91
This classifies the protein as stable

Atomic composition
Carbon 1883
Hydrogen 3060
Nitrogen 512
Oxygen 665
Sulfur 8
Total number of atoms 6128
Formula = C1883H3060N512O665S8

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 13325 M-1 cm-1
assuming NO Cys residues appear as half cystines 12950 M-1 cm-1
assuming all Cys residues are reduced 6990 M-1 cm-1
assuming all pairs of Cys residues form cystines 7240 M-1 cm-1

Aliphatic index
Aliphatic index 90.77

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000