RDH54/YBR073W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 41 4.3%
Arg[R] 48 5.0%
Asn[N] 56 5.8%
Asp[D] 56 5.8%
Cys[C] 17 1.8%
Gln[Q] 38 4.0%
Glu[E] 50 5.2%
Gly[G] 50 5.2%
His[H] 12 1.3%
Ile[I] 61 6.4%
Leu[L] 102 10.6%
Lys[K] 88 9.2%
Met[M] 24 2.5%
Phe[F] 38 4.0%
Pro[P] 42 4.4%
Ser[S] 83 8.7%
Thr[T] 72 7.5%
Trp[W] 6 0.6%
Tyr[Y] 25 2.6%
Val[V] 49 5.1%
Total Length 958

Instability index
Instability index (II) 41.99
This classifies the protein as unstable

Atomic composition
Carbon 4773
Hydrogen 7718
Nitrogen 1314
Oxygen 1445
Sulfur 41
Total number of atoms 15291
Formula = C4773H7718N1314O1445S41

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 71250 M-1 cm-1
assuming NO Cys residues appear as half cystines 70250 M-1 cm-1
assuming all Cys residues are reduced 70250 M-1 cm-1
assuming all pairs of Cys residues form cystines 71250 M-1 cm-1

Aliphatic index
Aliphatic index 85.47

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000