Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
38 |
5.7% |
| Arg[R] |
30 |
4.5% |
| Asn[N] |
30 |
4.5% |
| Asp[D] |
36 |
5.4% |
| Cys[C] |
9 |
1.3% |
| Gln[Q] |
27 |
4.0% |
| Glu[E] |
35 |
5.2% |
| Gly[G] |
38 |
5.7% |
| His[H] |
15 |
2.2% |
| Ile[I] |
44 |
6.6% |
| Leu[L] |
69 |
10.3% |
| Lys[K] |
47 |
7.0% |
| Met[M] |
14 |
2.1% |
| Phe[F] |
42 |
6.3% |
| Pro[P] |
27 |
4.0% |
| Ser[S] |
38 |
5.7% |
| Thr[T] |
41 |
6.1% |
| Trp[W] |
13 |
1.9% |
| Tyr[Y] |
34 |
5.1% |
| Val[V] |
42 |
6.3% |
| Total Length |
669 |
|
| Instability index (II) |
36.11 |
| This classifies the protein as stable |
|
| Carbon |
3541 |
| Hydrogen |
5428 |
| Nitrogen |
906 |
| Oxygen |
982 |
| Sulfur |
23 |
| Total number of atoms |
10880 |
| Formula = C3541H5428N906O982S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
122660 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
122160 M-1 cm-1 |
| assuming all Cys residues are reduced |
122160 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
122660 M-1 cm-1 |
|
|
| Codon Bias |
0.150 |
| Codon Adaptation Index |
0.194 |
| Frequency of Optimal Codons |
0.498 |
| Hydropathicity of Protein |
-0.173 |
| Aromaticity Score |
0.133 |
|
