Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
66 |
6.9% |
| Arg[R] |
49 |
5.1% |
| Asn[N] |
50 |
5.2% |
| Asp[D] |
48 |
5.0% |
| Cys[C] |
12 |
1.2% |
| Gln[Q] |
36 |
3.7% |
| Glu[E] |
50 |
5.2% |
| Gly[G] |
45 |
4.7% |
| His[H] |
21 |
2.2% |
| Ile[I] |
53 |
5.5% |
| Leu[L] |
89 |
9.2% |
| Lys[K] |
61 |
6.3% |
| Met[M] |
24 |
2.5% |
| Phe[F] |
57 |
5.9% |
| Pro[P] |
43 |
4.5% |
| Ser[S] |
89 |
9.2% |
| Thr[T] |
50 |
5.2% |
| Trp[W] |
17 |
1.8% |
| Tyr[Y] |
43 |
4.5% |
| Val[V] |
60 |
6.2% |
| Total Length |
963 |
|
| Instability index (II) |
43.48 |
| This classifies the protein as unstable |
|
| Carbon |
4973 |
| Hydrogen |
7656 |
| Nitrogen |
1316 |
| Oxygen |
1428 |
| Sulfur |
36 |
| Total number of atoms |
15409 |
| Formula = C4973H7656N1316O1428S36 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
158320 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
157570 M-1 cm-1 |
| assuming all Cys residues are reduced |
157570 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
158320 M-1 cm-1 |
|
|
| Codon Bias |
0.141 |
| Codon Adaptation Index |
0.172 |
| Frequency of Optimal Codons |
0.486 |
| Hydropathicity of Protein |
-0.261 |
| Aromaticity Score |
0.121 |
|
