CHS3/YBR023C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 59 5.1%
Arg[R] 46 3.9%
Asn[N] 59 5.1%
Asp[D] 84 7.2%
Cys[C] 22 1.9%
Gln[Q] 35 3.0%
Glu[E] 54 4.6%
Gly[G] 62 5.3%
His[H] 23 2.0%
Ile[I] 73 6.3%
Leu[L] 102 8.8%
Lys[K] 83 7.1%
Met[M] 30 2.6%
Phe[F] 56 4.8%
Pro[P] 55 4.7%
Ser[S] 105 9.0%
Thr[T] 73 6.3%
Trp[W] 23 2.0%
Tyr[Y] 41 3.5%
Val[V] 80 6.9%
Total Length 1165

Instability index
Instability index (II) 39.00
This classifies the protein as stable

Atomic composition
Carbon 5904
Hydrogen 9173
Nitrogen 1549
Oxygen 1755
Sulfur 52
Total number of atoms 18433
Formula = C5904H9173N1549O1755S52

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 188965 M-1 cm-1
assuming NO Cys residues appear as half cystines 187590 M-1 cm-1
assuming all Cys residues are reduced 187590 M-1 cm-1
assuming all pairs of Cys residues form cystines 188965 M-1 cm-1

Aliphatic index
Aliphatic index 83.56

Coding Region Translation Calculations
Codon Bias 0.114
Codon Adaptation Index 0.167
Frequency of Optimal Codons 0.478
Hydropathicity of Protein -0.267
Aromaticity Score 0.103