Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
51 |
8.1% |
| Arg[R] |
21 |
3.3% |
| Asn[N] |
29 |
4.6% |
| Asp[D] |
22 |
3.5% |
| Cys[C] |
16 |
2.5% |
| Gln[Q] |
15 |
2.4% |
| Glu[E] |
24 |
3.8% |
| Gly[G] |
37 |
5.8% |
| His[H] |
11 |
1.7% |
| Ile[I] |
46 |
7.3% |
| Leu[L] |
54 |
8.5% |
| Lys[K] |
29 |
4.6% |
| Met[M] |
15 |
2.4% |
| Phe[F] |
49 |
7.7% |
| Pro[P] |
26 |
4.1% |
| Ser[S] |
64 |
10.1% |
| Thr[T] |
25 |
3.9% |
| Trp[W] |
22 |
3.5% |
| Tyr[Y] |
33 |
5.2% |
| Val[V] |
44 |
7.0% |
| Total Length |
633 |
|
| Instability index (II) |
39.43 |
| This classifies the protein as stable |
|
| Carbon |
3337 |
| Hydrogen |
4963 |
| Nitrogen |
813 |
| Oxygen |
892 |
| Sulfur |
31 |
| Total number of atoms |
10036 |
| Formula = C3337H4963N813O892S31 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
171170 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
170170 M-1 cm-1 |
| assuming all Cys residues are reduced |
170170 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
171170 M-1 cm-1 |
|
|
| Codon Bias |
0.182 |
| Codon Adaptation Index |
0.186 |
| Frequency of Optimal Codons |
0.513 |
| Hydropathicity of Protein |
0.235 |
| Aromaticity Score |
0.164 |
|
