Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
44 |
6.3% |
| Arg[R] |
28 |
4.0% |
| Asn[N] |
48 |
6.9% |
| Asp[D] |
40 |
5.7% |
| Cys[C] |
10 |
1.4% |
| Gln[Q] |
21 |
3.0% |
| Glu[E] |
44 |
6.3% |
| Gly[G] |
54 |
7.7% |
| His[H] |
14 |
2.0% |
| Ile[I] |
44 |
6.3% |
| Leu[L] |
57 |
8.2% |
| Lys[K] |
43 |
6.2% |
| Met[M] |
9 |
1.3% |
| Phe[F] |
30 |
4.3% |
| Pro[P] |
31 |
4.4% |
| Ser[S] |
42 |
6.0% |
| Thr[T] |
45 |
6.4% |
| Trp[W] |
6 |
0.9% |
| Tyr[Y] |
39 |
5.6% |
| Val[V] |
50 |
7.2% |
| Total Length |
699 |
|
| Instability index (II) |
27.26 |
| This classifies the protein as stable |
|
| Carbon |
3506 |
| Hydrogen |
5413 |
| Nitrogen |
929 |
| Oxygen |
1063 |
| Sulfur |
19 |
| Total number of atoms |
10930 |
| Formula = C3506H5413N929O1063S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
91735 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
91110 M-1 cm-1 |
| assuming all Cys residues are reduced |
91110 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
91735 M-1 cm-1 |
|
|
| Codon Bias |
0.142 |
| Codon Adaptation Index |
0.186 |
| Frequency of Optimal Codons |
0.496 |
| Hydropathicity of Protein |
-0.338 |
| Aromaticity Score |
0.107 |
|
