Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
56 |
6.1% |
| Arg[R] |
23 |
2.5% |
| Asn[N] |
57 |
6.2% |
| Asp[D] |
70 |
7.6% |
| Cys[C] |
16 |
1.7% |
| Gln[Q] |
37 |
4.0% |
| Glu[E] |
64 |
7.0% |
| Gly[G] |
35 |
3.8% |
| His[H] |
19 |
2.1% |
| Ile[I] |
76 |
8.3% |
| Leu[L] |
111 |
12.1% |
| Lys[K] |
44 |
4.8% |
| Met[M] |
28 |
3.1% |
| Phe[F] |
47 |
5.1% |
| Pro[P] |
37 |
4.0% |
| Ser[S] |
68 |
7.4% |
| Thr[T] |
46 |
5.0% |
| Trp[W] |
12 |
1.3% |
| Tyr[Y] |
21 |
2.3% |
| Val[V] |
51 |
5.6% |
| Total Length |
918 |
|
| Instability index (II) |
43.25 |
| This classifies the protein as unstable |
|
| Carbon |
4649 |
| Hydrogen |
7261 |
| Nitrogen |
1175 |
| Oxygen |
1416 |
| Sulfur |
44 |
| Total number of atoms |
14545 |
| Formula = C4649H7261N1175O1416S44 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
98290 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
97290 M-1 cm-1 |
| assuming all Cys residues are reduced |
97290 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
98290 M-1 cm-1 |
|
|
| Codon Bias |
0.041 |
| Codon Adaptation Index |
0.144 |
| Frequency of Optimal Codons |
0.438 |
| Hydropathicity of Protein |
-0.031 |
| Aromaticity Score |
0.087 |
|
