Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
34 |
5.7% |
| Arg[R] |
17 |
2.8% |
| Asn[N] |
35 |
5.9% |
| Asp[D] |
48 |
8.0% |
| Cys[C] |
7 |
1.2% |
| Gln[Q] |
9 |
1.5% |
| Glu[E] |
34 |
5.7% |
| Gly[G] |
35 |
5.9% |
| His[H] |
11 |
1.8% |
| Ile[I] |
29 |
4.9% |
| Leu[L] |
60 |
10.1% |
| Lys[K] |
57 |
9.5% |
| Met[M] |
10 |
1.7% |
| Phe[F] |
34 |
5.7% |
| Pro[P] |
25 |
4.2% |
| Ser[S] |
43 |
7.2% |
| Thr[T] |
36 |
6.0% |
| Trp[W] |
4 |
0.7% |
| Tyr[Y] |
36 |
6.0% |
| Val[V] |
33 |
5.5% |
| Total Length |
597 |
|
| Instability index (II) |
30.14 |
| This classifies the protein as stable |
|
| Carbon |
3071 |
| Hydrogen |
4715 |
| Nitrogen |
775 |
| Oxygen |
921 |
| Sulfur |
17 |
| Total number of atoms |
9499 |
| Formula = C3071H4715N775O921S17 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
76015 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
75640 M-1 cm-1 |
| assuming all Cys residues are reduced |
75640 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
76015 M-1 cm-1 |
|
|
| Codon Bias |
0.131 |
| Codon Adaptation Index |
0.170 |
| Frequency of Optimal Codons |
0.489 |
| Hydropathicity of Protein |
-0.417 |
| Aromaticity Score |
0.124 |
|
