Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
42 |
8.5% |
| Arg[R] |
15 |
3.0% |
| Asn[N] |
23 |
4.6% |
| Asp[D] |
19 |
3.8% |
| Cys[C] |
11 |
2.2% |
| Gln[Q] |
9 |
1.8% |
| Glu[E] |
36 |
7.2% |
| Gly[G] |
39 |
7.8% |
| His[H] |
7 |
1.4% |
| Ile[I] |
30 |
6.0% |
| Leu[L] |
41 |
8.2% |
| Lys[K] |
37 |
7.4% |
| Met[M] |
9 |
1.8% |
| Phe[F] |
24 |
4.8% |
| Pro[P] |
26 |
5.2% |
| Ser[S] |
33 |
6.6% |
| Thr[T] |
35 |
7.0% |
| Trp[W] |
4 |
0.8% |
| Tyr[Y] |
17 |
3.4% |
| Val[V] |
40 |
8.0% |
| Total Length |
497 |
|
| Instability index (II) |
30.28 |
| This classifies the protein as stable |
|
| Carbon |
2437 |
| Hydrogen |
3837 |
| Nitrogen |
629 |
| Oxygen |
725 |
| Sulfur |
20 |
| Total number of atoms |
7648 |
| Formula = C2437H3837N629O725S20 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
47955 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
47330 M-1 cm-1 |
| assuming all Cys residues are reduced |
47330 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
47955 M-1 cm-1 |
|
|
| Codon Bias |
0.201 |
| Codon Adaptation Index |
0.195 |
| Frequency of Optimal Codons |
0.525 |
| Hydropathicity of Protein |
-0.053 |
| Aromaticity Score |
0.091 |
|
