PKC1/YBL105C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 64 5.6%
Arg[R] 56 4.9%
Asn[N] 58 5.0%
Asp[D] 72 6.3%
Cys[C] 24 2.1%
Gln[Q] 72 6.3%
Glu[E] 87 7.6%
Gly[G] 55 4.8%
His[H] 30 2.6%
Ile[I] 71 6.2%
Leu[L] 98 8.5%
Lys[K] 98 8.5%
Met[M] 25 2.2%
Phe[F] 48 4.2%
Pro[P] 49 4.3%
Ser[S] 86 7.5%
Thr[T] 73 6.3%
Trp[W] 13 1.1%
Tyr[Y] 29 2.5%
Val[V] 43 3.7%
Total Length 1151

Instability index
Instability index (II) 47.60
This classifies the protein as unstable

Atomic composition
Carbon 5778
Hydrogen 9136
Nitrogen 1620
Oxygen 1788
Sulfur 49
Total number of atoms 18371
Formula = C5778H9136N1620O1788S49

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 116085 M-1 cm-1
assuming NO Cys residues appear as half cystines 114710 M-1 cm-1
assuming all Cys residues are reduced 114710 M-1 cm-1
assuming all pairs of Cys residues form cystines 116210 M-1 cm-1

Aliphatic index
Aliphatic index 73.66

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000