Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
59 |
5.7% |
| Arg[R] |
64 |
6.2% |
| Asn[N] |
85 |
8.2% |
| Asp[D] |
75 |
7.2% |
| Cys[C] |
23 |
2.2% |
| Gln[Q] |
29 |
2.8% |
| Glu[E] |
62 |
6.0% |
| Gly[G] |
60 |
5.8% |
| His[H] |
19 |
1.8% |
| Ile[I] |
72 |
6.9% |
| Leu[L] |
85 |
8.2% |
| Lys[K] |
57 |
5.5% |
| Met[M] |
23 |
2.2% |
| Phe[F] |
33 |
3.2% |
| Pro[P] |
36 |
3.5% |
| Ser[S] |
97 |
9.3% |
| Thr[T] |
55 |
5.3% |
| Trp[W] |
19 |
1.8% |
| Tyr[Y] |
40 |
3.9% |
| Val[V] |
45 |
4.3% |
| Total Length |
1038 |
|
| Instability index (II) |
39.55 |
| This classifies the protein as stable |
|
| Carbon |
5140 |
| Hydrogen |
8036 |
| Nitrogen |
1458 |
| Oxygen |
1619 |
| Sulfur |
46 |
| Total number of atoms |
16299 |
| Formula = C5140H8036N1458O1619S46 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
165475 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
164100 M-1 cm-1 |
| assuming all Cys residues are reduced |
164100 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
165475 M-1 cm-1 |
|
|
| Codon Bias |
0.064 |
| Codon Adaptation Index |
0.142 |
| Frequency of Optimal Codons |
0.445 |
| Hydropathicity of Protein |
-0.559 |
| Aromaticity Score |
0.089 |
|
