RTG3/YBL103C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 25 5.1%
Arg[R] 22 4.5%
Asn[N] 40 8.2%
Asp[D] 26 5.3%
Cys[C] 0 0.0%
Gln[Q] 23 4.7%
Glu[E] 37 7.6%
Gly[G] 27 5.6%
His[H] 18 3.7%
Ile[I] 23 4.7%
Leu[L] 45 9.3%
Lys[K] 24 4.9%
Met[M] 14 2.9%
Phe[F] 13 2.7%
Pro[P] 27 5.6%
Ser[S] 65 13.4%
Thr[T] 27 5.6%
Trp[W] 1 0.2%
Tyr[Y] 16 3.3%
Val[V] 13 2.7%
Total Length 486

Instability index
Instability index (II) 54.66
This classifies the protein as unstable

Atomic composition
Carbon 2330
Hydrogen 3664
Nitrogen 676
Oxygen 784
Sulfur 14
Total number of atoms 7468
Formula = C2330H3664N676O784S14

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 29340 M-1 cm-1

Aliphatic index
Aliphatic index 67.47

Coding Region Translation Calculations
Codon Bias 0.079
Codon Adaptation Index 0.147
Frequency of Optimal Codons 0.444
Hydropathicity of Protein -0.824
Aromaticity Score 0.062