Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
59 |
9.1% |
| Arg[R] |
34 |
5.2% |
| Asn[N] |
32 |
4.9% |
| Asp[D] |
51 |
7.9% |
| Cys[C] |
2 |
0.3% |
| Gln[Q] |
20 |
3.1% |
| Glu[E] |
49 |
7.6% |
| Gly[G] |
53 |
8.2% |
| His[H] |
6 |
0.9% |
| Ile[I] |
39 |
6.0% |
| Leu[L] |
46 |
7.1% |
| Lys[K] |
46 |
7.1% |
| Met[M] |
9 |
1.4% |
| Phe[F] |
27 |
4.2% |
| Pro[P] |
25 |
3.9% |
| Ser[S] |
44 |
6.8% |
| Thr[T] |
54 |
8.3% |
| Trp[W] |
1 |
0.2% |
| Tyr[Y] |
13 |
2.0% |
| Val[V] |
39 |
6.0% |
| Total Length |
649 |
|
| Instability index (II) |
29.77 |
| This classifies the protein as stable |
|
| Carbon |
3076 |
| Hydrogen |
4928 |
| Nitrogen |
862 |
| Oxygen |
1013 |
| Sulfur |
11 |
| Total number of atoms |
9890 |
| Formula = C3076H4928N862O1013S11 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
24995 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
24870 M-1 cm-1 |
| assuming all Cys residues are reduced |
24870 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
24995 M-1 cm-1 |
|
|
| Codon Bias |
0.155 |
| Codon Adaptation Index |
0.177 |
| Frequency of Optimal Codons |
0.496 |
| Hydropathicity of Protein |
-0.489 |
| Aromaticity Score |
0.063 |
|
