PRS4/YBL068W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 31 9.5%
Arg[R] 14 4.3%
Asn[N] 16 4.9%
Asp[D] 24 7.4%
Cys[C] 5 1.5%
Gln[Q] 10 3.1%
Glu[E] 17 5.2%
Gly[G] 16 4.9%
His[H] 10 3.1%
Ile[I] 29 8.9%
Leu[L] 32 9.8%
Lys[K] 21 6.4%
Met[M] 7 2.1%
Phe[F] 9 2.8%
Pro[P] 12 3.7%
Ser[S] 23 7.1%
Thr[T] 19 5.8%
Trp[W] 0 0.0%
Tyr[Y] 7 2.1%
Val[V] 24 7.4%
Total Length 326

Instability index
Instability index (II) 34.11
This classifies the protein as stable

Atomic composition
Carbon 1575
Hydrogen 2561
Nitrogen 435
Oxygen 484
Sulfur 12
Total number of atoms 5067
Formula = C1575H2561N435O484S12

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 10680 M-1 cm-1
assuming NO Cys residues appear as half cystines 10430 M-1 cm-1
assuming all Cys residues are reduced 10430 M-1 cm-1
assuming all pairs of Cys residues form cystines 10680 M-1 cm-1

Aliphatic index
Aliphatic index 103.83

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000