Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
24 |
2.9% |
| Arg[R] |
55 |
6.6% |
| Asn[N] |
66 |
7.9% |
| Asp[D] |
59 |
7.1% |
| Cys[C] |
11 |
1.3% |
| Gln[Q] |
24 |
2.9% |
| Glu[E] |
75 |
9.0% |
| Gly[G] |
30 |
3.6% |
| His[H] |
17 |
2.0% |
| Ile[I] |
58 |
7.0% |
| Leu[L] |
66 |
7.9% |
| Lys[K] |
69 |
8.3% |
| Met[M] |
12 |
1.4% |
| Phe[F] |
37 |
4.5% |
| Pro[P] |
31 |
3.7% |
| Ser[S] |
67 |
8.1% |
| Thr[T] |
49 |
5.9% |
| Trp[W] |
12 |
1.4% |
| Tyr[Y] |
35 |
4.2% |
| Val[V] |
34 |
4.1% |
| Total Length |
831 |
|
| Instability index (II) |
56.65 |
| This classifies the protein as unstable |
|
| Carbon |
4312 |
| Hydrogen |
6723 |
| Nitrogen |
1201 |
| Oxygen |
1341 |
| Sulfur |
23 |
| Total number of atoms |
13600 |
| Formula = C4312H6723N1201O1341S23 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
118775 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
118150 M-1 cm-1 |
| assuming all Cys residues are reduced |
118150 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
118775 M-1 cm-1 |
|
|
| Codon Bias |
-0.007 |
| Codon Adaptation Index |
0.132 |
| Frequency of Optimal Codons |
0.418 |
| Hydropathicity of Protein |
-0.853 |
| Aromaticity Score |
0.101 |
|
