EDE1/YBL047C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 125 9.1%
Arg[R] 38 2.8%
Asn[N] 111 8.0%
Asp[D] 81 5.9%
Cys[C] 3 0.2%
Gln[Q] 107 7.7%
Glu[E] 111 8.0%
Gly[G] 51 3.7%
His[H] 21 1.5%
Ile[I] 53 3.8%
Leu[L] 117 8.5%
Lys[K] 71 5.1%
Met[M] 20 1.4%
Phe[F] 46 3.3%
Pro[P] 81 5.9%
Ser[S] 148 10.7%
Thr[T] 106 7.7%
Trp[W] 8 0.6%
Tyr[Y] 14 1.0%
Val[V] 69 5.0%
Total Length 1381

Instability index
Instability index (II) 48.12
This classifies the protein as unstable

Atomic composition
Carbon 6490
Hydrogen 10294
Nitrogen 1834
Oxygen 2252
Sulfur 23
Total number of atoms 20893
Formula = C6490H10294N1834O2252S23

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 64985 M-1 cm-1
assuming NO Cys residues appear as half cystines 64860 M-1 cm-1
assuming all Cys residues are reduced 64860 M-1 cm-1
assuming all pairs of Cys residues form cystines 64985 M-1 cm-1

Aliphatic index
Aliphatic index 71.55

Coding Region Translation Calculations
Codon Bias 0.192
Codon Adaptation Index 0.197
Frequency of Optimal Codons 0.517
Hydropathicity of Protein -0.685
Aromaticity Score 0.049