Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
49 |
7.7% |
| Arg[R] |
21 |
3.3% |
| Asn[N] |
35 |
5.5% |
| Asp[D] |
30 |
4.7% |
| Cys[C] |
11 |
1.7% |
| Gln[Q] |
11 |
1.7% |
| Glu[E] |
24 |
3.8% |
| Gly[G] |
41 |
6.4% |
| His[H] |
5 |
0.8% |
| Ile[I] |
58 |
9.1% |
| Leu[L] |
56 |
8.8% |
| Lys[K] |
34 |
5.3% |
| Met[M] |
10 |
1.6% |
| Phe[F] |
43 |
6.7% |
| Pro[P] |
22 |
3.4% |
| Ser[S] |
52 |
8.1% |
| Thr[T] |
40 |
6.3% |
| Trp[W] |
20 |
3.1% |
| Tyr[Y] |
36 |
5.6% |
| Val[V] |
41 |
6.4% |
| Total Length |
639 |
|
| Instability index (II) |
30.50 |
| This classifies the protein as stable |
|
| Carbon |
3353 |
| Hydrogen |
5054 |
| Nitrogen |
812 |
| Oxygen |
922 |
| Sulfur |
21 |
| Total number of atoms |
10162 |
| Formula = C3353H5054N812O922S21 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
164265 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
163640 M-1 cm-1 |
| assuming all Cys residues are reduced |
163640 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
164265 M-1 cm-1 |
|
|
| Codon Bias |
0.174 |
| Codon Adaptation Index |
0.168 |
| Frequency of Optimal Codons |
0.516 |
| Hydropathicity of Protein |
0.191 |
| Aromaticity Score |
0.155 |
|
