PET9/YBL030C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 34 10.7%
Arg[R] 15 4.7%
Asn[N] 9 2.8%
Asp[D] 14 4.4%
Cys[C] 4 1.3%
Gln[Q] 10 3.1%
Glu[E] 8 2.5%
Gly[G] 35 11.0%
His[H] 0 0.0%
Ile[I] 13 4.1%
Leu[L] 34 10.7%
Lys[K] 27 8.5%
Met[M] 10 3.1%
Phe[F] 20 6.3%
Pro[P] 10 3.1%
Ser[S] 23 7.2%
Thr[T] 15 4.7%
Trp[W] 3 0.9%
Tyr[Y] 13 4.1%
Val[V] 21 6.6%
Total Length 318

Instability index
Instability index (II) 26.28
This classifies the protein as stable

Atomic composition
Carbon 1564
Hydrogen 2474
Nitrogen 412
Oxygen 433
Sulfur 14
Total number of atoms 4897
Formula = C1564H2474N412O433S14

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 36120 M-1 cm-1
assuming NO Cys residues appear as half cystines 35870 M-1 cm-1
assuming all Cys residues are reduced 35870 M-1 cm-1
assuming all pairs of Cys residues form cystines 36120 M-1 cm-1

Aliphatic index
Aliphatic index 87.48

Coding Region Translation Calculations
Codon Bias 0.622
Codon Adaptation Index 0.538
Frequency of Optimal Codons 0.764
Hydropathicity of Protein 0.086
Aromaticity Score 0.113