LSM2/YBL026W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 1 1.1%
Arg[R] 6 6.3%
Asn[N] 7 7.4%
Asp[D] 7 7.4%
Cys[C] 1 1.1%
Gln[Q] 4 4.2%
Glu[E] 6 6.3%
Gly[G] 3 3.2%
His[H] 1 1.1%
Ile[I] 5 5.3%
Leu[L] 11 11.6%
Lys[K] 8 8.4%
Met[M] 3 3.2%
Phe[F] 6 6.3%
Pro[P] 1 1.1%
Ser[S] 5 5.3%
Thr[T] 7 7.4%
Trp[W] 0 0.0%
Tyr[Y] 3 3.2%
Val[V] 10 10.5%
Total Length 95

Instability index
Instability index (II) 42.92
This classifies the protein as unstable

Atomic composition
Carbon 498
Hydrogen 802
Nitrogen 134
Oxygen 148
Sulfur 4
Total number of atoms 1586
Formula = C498H802N134O148S4

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 4470 M-1 cm-1
assuming NO Cys residues appear as half cystines 4470 M-1 cm-1
assuming all Cys residues are reduced 4470 M-1 cm-1
assuming all pairs of Cys residues form cystines 4470 M-1 cm-1

Aliphatic index
Aliphatic index 97.26

Coding Region Translation Calculations
Codon Bias 0.169
Codon Adaptation Index 0.169
Frequency of Optimal Codons 0.522
Hydropathicity of Protein -0.294
Aromaticity Score 0.095