Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
52 |
6.0% |
| Arg[R] |
77 |
8.9% |
| Asn[N] |
43 |
5.0% |
| Asp[D] |
69 |
7.9% |
| Cys[C] |
9 |
1.0% |
| Gln[Q] |
32 |
3.7% |
| Glu[E] |
86 |
9.9% |
| Gly[G] |
46 |
5.3% |
| His[H] |
17 |
2.0% |
| Ile[I] |
55 |
6.3% |
| Leu[L] |
73 |
8.4% |
| Lys[K] |
38 |
4.4% |
| Met[M] |
18 |
2.1% |
| Phe[F] |
25 |
2.9% |
| Pro[P] |
36 |
4.1% |
| Ser[S] |
68 |
7.8% |
| Thr[T] |
36 |
4.1% |
| Trp[W] |
3 |
0.3% |
| Tyr[Y] |
26 |
3.0% |
| Val[V] |
59 |
6.8% |
| Total Length |
868 |
|
| Instability index (II) |
53.14 |
| This classifies the protein as unstable |
|
| Carbon |
4278 |
| Hydrogen |
6839 |
| Nitrogen |
1249 |
| Oxygen |
1384 |
| Sulfur |
27 |
| Total number of atoms |
13777 |
| Formula = C4278H6839N1249O1384S27 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
55740 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
55240 M-1 cm-1 |
| assuming all Cys residues are reduced |
55240 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
55740 M-1 cm-1 |
|
|
| Codon Bias |
0.150 |
| Codon Adaptation Index |
0.175 |
| Frequency of Optimal Codons |
0.489 |
| Hydropathicity of Protein |
-0.637 |
| Aromaticity Score |
0.062 |
|
