Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
76 |
6.7% |
| Arg[R] |
51 |
4.5% |
| Asn[N] |
46 |
4.1% |
| Asp[D] |
84 |
7.4% |
| Cys[C] |
7 |
0.6% |
| Gln[Q] |
40 |
3.5% |
| Glu[E] |
102 |
9.0% |
| Gly[G] |
55 |
4.9% |
| His[H] |
19 |
1.7% |
| Ile[I] |
72 |
6.4% |
| Leu[L] |
95 |
8.4% |
| Lys[K] |
107 |
9.4% |
| Met[M] |
23 |
2.0% |
| Phe[F] |
36 |
3.2% |
| Pro[P] |
54 |
4.8% |
| Ser[S] |
91 |
8.0% |
| Thr[T] |
70 |
6.2% |
| Trp[W] |
7 |
0.6% |
| Tyr[Y] |
29 |
2.6% |
| Val[V] |
69 |
6.1% |
| Total Length |
1133 |
|
| Instability index (II) |
40.91 |
| This classifies the protein as unstable |
|
| Carbon |
5598 |
| Hydrogen |
8984 |
| Nitrogen |
1524 |
| Oxygen |
1782 |
| Sulfur |
30 |
| Total number of atoms |
17918 |
| Formula = C5598H8984N1524O1782S30 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
82085 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
81710 M-1 cm-1 |
| assuming all Cys residues are reduced |
81710 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
82085 M-1 cm-1 |
|
|
| Codon Bias |
0.176 |
| Codon Adaptation Index |
0.186 |
| Frequency of Optimal Codons |
0.515 |
| Hydropathicity of Protein |
-0.583 |
| Aromaticity Score |
0.064 |
|
