Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
72 |
4.6% |
| Arg[R] |
54 |
3.4% |
| Asn[N] |
92 |
5.8% |
| Asp[D] |
131 |
8.3% |
| Cys[C] |
37 |
2.3% |
| Gln[Q] |
42 |
2.7% |
| Glu[E] |
115 |
7.3% |
| Gly[G] |
112 |
7.1% |
| His[H] |
24 |
1.5% |
| Ile[I] |
98 |
6.2% |
| Leu[L] |
108 |
6.8% |
| Lys[K] |
109 |
6.9% |
| Met[M] |
28 |
1.8% |
| Phe[F] |
99 |
6.3% |
| Pro[P] |
57 |
3.6% |
| Ser[S] |
131 |
8.3% |
| Thr[T] |
112 |
7.1% |
| Trp[W] |
17 |
1.1% |
| Tyr[Y] |
62 |
3.9% |
| Val[V] |
79 |
5.0% |
| Total Length |
1579 |
|
| Instability index (II) |
35.40 |
| This classifies the protein as stable |
|
| Carbon |
7883 |
| Hydrogen |
12041 |
| Nitrogen |
2049 |
| Oxygen |
2511 |
| Sulfur |
65 |
| Total number of atoms |
24549 |
| Formula = C7883H12041N2049O2511S65 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
188130 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
185880 M-1 cm-1 |
| assuming all Cys residues are reduced |
185880 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
188130 M-1 cm-1 |
|
|
| Codon Bias |
0.061 |
| Codon Adaptation Index |
0.163 |
| Frequency of Optimal Codons |
0.457 |
| Hydropathicity of Protein |
-0.478 |
| Aromaticity Score |
0.113 |
|
